Journal: Connective tissue research

Article Title: S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway.

doi: 10.1080/03008207.2024.2310852

Figure Lengend Snippet: Figure 1. Dysregulated secreted proteins in OA SF and IL-1β-induced chondrocyte S100A12 upregulation. (A) Semi-quantitative antibody arrays were used to outline the proteome of SF. (B) Scatter plot of protein expression levels. (C-D) GO and KEGG analysis on DEPs. (E, G) the expression of S100A12 in mRNA levels increased in chondrocytes under IL-1β stimulation. (F, H) the expression of S100A12 in protein levels increased in chondrocytes under IL-1β stimulation. (I, J) the extracellular S100A12 of chondrocyte supernatant was detected by ELISA under IL-1β stimulation. Data represent the mean ± standard deviation (3 independently repeated experiments); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant (Student t test). BFA, Brefeldin A.

Article Snippet: To induce M0 macrophages in vitro, THP-1 cells were treated with phorbol 12- myristate 13-acetate (PMA) (100 ng/mL) for 48 h. Then, the differentiated M0 macrophages were stimulated with recombinant human S100A12 protein (ABclonal, RP00009) in the absence or presence of BAY 11–7082 pretreatment (5 μM for 1 h) to verify the effect of S100A12 on macrophage polarization.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Connective tissue research

Article Title: S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway.

doi: 10.1080/03008207.2024.2310852

Figure Lengend Snippet: Figure 2. S100A12 is involved in the pathology of OA synovitis. (A) S100A12 levels in different tissues of OA or non-OA joints. (B) HE and S100A12 IHC staining of OA and non-OA synovium (C, D) statistical analysis of synovitis scores and S100A12 IHC in OA and non-OA synovium. Data represent the mean ± standard deviation (3 independently repeated experiments); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant (Student t test). (E) Correlation analysis between synovitis score and S100A12 concentration (Pearson).

Article Snippet: To induce M0 macrophages in vitro, THP-1 cells were treated with phorbol 12- myristate 13-acetate (PMA) (100 ng/mL) for 48 h. Then, the differentiated M0 macrophages were stimulated with recombinant human S100A12 protein (ABclonal, RP00009) in the absence or presence of BAY 11–7082 pretreatment (5 μM for 1 h) to verify the effect of S100A12 on macrophage polarization.

Techniques: Immunohistochemistry, Standard Deviation, Concentration Assay

Journal: Connective tissue research

Article Title: S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway.

doi: 10.1080/03008207.2024.2310852

Figure Lengend Snippet: Figure 4. S100A12 promotes macrophage M1 polarization via the NF-κB signaling pathway. (A) STRING prediction for S100A12. (B) Western blot analysis of protein levels in THP-1-derived macrophages treated with exogenous S100A12 (2ug/ml). (C, D) Representative immunofluorescence images of THP-1-derived macrophages treated with indicated conditions and quantitative analysis of p65 nuclear translocation. Data represent the mean ± standard deviation (3 independently repeated experiments); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant (Student t test). (E-G) Western blot and quantification analysis of protein levels in THP-1-derived macrophages with or without BAY 11–7082 pretreated. BFA, Brefeldin A. Data represent the mean ± standard deviation (3 independently repeated experiments); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant (ANOVA).

Article Snippet: To induce M0 macrophages in vitro, THP-1 cells were treated with phorbol 12- myristate 13-acetate (PMA) (100 ng/mL) for 48 h. Then, the differentiated M0 macrophages were stimulated with recombinant human S100A12 protein (ABclonal, RP00009) in the absence or presence of BAY 11–7082 pretreatment (5 μM for 1 h) to verify the effect of S100A12 on macrophage polarization.

Techniques: Western Blot, Derivative Assay, Immunofluorescence, Translocation Assay, Standard Deviation

Journal: Connective tissue research

Article Title: S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway.

doi: 10.1080/03008207.2024.2310852

Figure Lengend Snippet: Figure 5. Construction of the ceRNA network regulating S100A12. (A) The potential miRnas targeting S100A12 were predicted using miRDB, miRmap, miRwalk, and TargetScan databases. Several common miRnas were obtained through screening with a venn diagram. The horizontal bar represents the number of elements shared in one or more lists. For example, 115 elements exclusively exist in a single list, while 30 elements coexist in 2 lists. (B) LncACTdb was used to predict lncRnas that may bind to these miRnas, and the network was visualized. (C) Venn diagram of predicted lncRnas and lncRnas detectable by GPL20844. (D) Volcano plot for differential analysis. (E) Venn diagram of differentially expressed lncRnas and lncRnas positively correlated with S100A12. (F,G) correlation analysis between LINC00894 expression and S100A12 expression (Pearson).

Article Snippet: To induce M0 macrophages in vitro, THP-1 cells were treated with phorbol 12- myristate 13-acetate (PMA) (100 ng/mL) for 48 h. Then, the differentiated M0 macrophages were stimulated with recombinant human S100A12 protein (ABclonal, RP00009) in the absence or presence of BAY 11–7082 pretreatment (5 μM for 1 h) to verify the effect of S100A12 on macrophage polarization.

Techniques: Expressing

Journal: Connective tissue research

Article Title: S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway.

doi: 10.1080/03008207.2024.2310852

Figure Lengend Snippet: Figure 6. The schematic diagram summarizes the content of this study and the role of S100A12 in OA.

Article Snippet: To induce M0 macrophages in vitro, THP-1 cells were treated with phorbol 12- myristate 13-acetate (PMA) (100 ng/mL) for 48 h. Then, the differentiated M0 macrophages were stimulated with recombinant human S100A12 protein (ABclonal, RP00009) in the absence or presence of BAY 11–7082 pretreatment (5 μM for 1 h) to verify the effect of S100A12 on macrophage polarization.

Techniques:

Journal: Disease markers

Article Title: Fecal S100A12 in Healthy Infants and Children

doi: 10.1155/2013/873582

Figure Lengend Snippet: Faecal S100A12 concentrations in 56 healthy infants and children. Serial stools collected from the first day of life (meconium) to 6 months of age from 7 healthy infants (Population 1) and single stools collected from 49 children (Population 2) were utilised to measure faecal S100A12 concentrations by immunoassay.

Article Snippet: Dilutions of recombinant S100A12 (Recombinant Human EN-RAGE/S100A12, R&D Systems, Minneapolis, MN, USA) were added (100 μ L/well) in order to create a standard curve.

Techniques:

Journal: Disease markers

Article Title: Fecal S100A12 in Healthy Infants and Children

doi: 10.1155/2013/873582

Figure Lengend Snippet: Measurement of fecal S100A12 infants and children. Repeated fecal samples were collected from seven term infants over the first six months of life (a). Single stool samples were collected from 49 healthy infants and children (b). S100A12 concentrations were measured by immunoassay. Only five samples (all in infants) were above the cut-off of 10 mg/kg.

Article Snippet: Dilutions of recombinant S100A12 (Recombinant Human EN-RAGE/S100A12, R&D Systems, Minneapolis, MN, USA) were added (100 μ L/well) in order to create a standard curve.

Techniques: